Unit of competency Outline

Date retreived
23/07/2026 7:15 AM AWST

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Prepare tissue and cell cultures

Prepare tissue and cell cultures

Unit of competency
National Code
MSL974011
State Code
AWO01
TGA Status
Replaced
DTWD Status
Replaced
Current Release Number
1.00
Current Release Date
01/03/2016
View on TGA
State Implementation and Classification
Approved Date
16/08/2016
Field of Education
019909 - Laboratory Technology
Original Release Date
16/08/2016
Nominal Hours
40
Description
This unit of competency covers the ability to prepare primary tissue cultures for applications, such as maintenance of animal cell lines and propagation of plants by tissue culture, and basic subculture procedures. Personnel are required to manipulate equipment and materials and samples to prevent contamination at all preparation stages. They will have ready access to workplace procedures and will work under direct supervision.This unit of competency is applicable to technical assistants working in laboratories in the biomedical, environmental, biotechnology and education industry sectors.While no specific licensing or certification requirements apply to this unit at the time of publication, laboratory operations are governed by relevant legislation, regulations and/or external accreditation requirements. Local requirements should be checked.
Notes
Elements and Performance Criteria
1 Work safely according to the legal and regulatory framework
  • 1.1 Ensure work practices and personal actions conform to regulations, codes, guidelines and workplace quality assurance procedures
  • 1.2 Identify hazards and workplace controls associated with the sample, preparation methods, reagents and equipment
  • 1.3 Select, fit and use personal protective equipment (PPE)
  • 1.4 Address hazards and incidents as they arise
  • 1.5 Maintain a chain of custody, traceable to the worker, for all cells and tissues
2 Prepare and test cell and tissue culture media
  • 2.1 Select and confirm media specifications and processes/methods
  • 2.2 Prepare culture media to suit the application
  • 2.3 Sterilise culture media and check for sterility
  • 2.4 Perform quality control checks to ensure that culture media is fit for purpose
  • 2.5 Store culture media in accordance with specifications
3 Prepare tissue or cell cultures
  • 3.1 Select tissue/cell sample to optimise growth and prepare it for culture
  • 3.2 Add specified growth agents and/or nutrients
  • 3.3 Inoculate culture medium using aseptic techniques
4 Monitor tissue or cell culture
  • 4.1 Incubate culture in specified conditions
  • 4.2 Monitor growth of culture and record appearance and characteristics
  • 4.3 Report presence or absence of contamination
  • 4.4 Subculture the culture to continue the cell line
  • 4.5 Dispose of biohazardous and other laboratory waste safely
5 Maintain records
  • 5.1 Maintain records of batches of media and test data
  • 5.2 Ensure records of tissue cultures are retrievable, legible and accurate
  • 5.3 Ensure records conform to information management, records, quality system and legal requirements
No information
No information
Replaces
State Code National Code Title Type
WA727 MSL974011A Prepare tissue and cell cultures Unit of competency
Replaced By
State Code National Code Title Type
OAC10 MSL974025 Prepare tissue and cell cultures Unit of competency