Unit of competency Outline

Date retreived
23/07/2026 4:45 AM AWST

Whilst all efforts are made to provide accurate and timely information from the relevant source/documentation, please be aware that the information supplied may not be the most current version. The accuracy of the detail has not been confirmed by the Department and therefore should not be relied upon without first confirming the contents.

Prepare tissue or cell cultures

Prepare tissue or cell cultures

Unit of competency
National Code
MSL974035
State Code
OEX75
TGA Status
Current
DTWD Status
Approved
Current Release Number
1.00
Current Release Date
20/12/2022
View on TGA
State Implementation and Classification
Approved Date
24/05/2023
Field of Education
019909 - Laboratory Technology
Original Release Date
24/05/2023
Nominal Hours
40
Description
This unit of competency describes the skills and knowledge to prepare primary tissue cultures for applications, such as maintenance of animal cell lines and propagation of plants by tissue culture, and basic subculture procedures. Personnel are required to manipulate equipment and materials and samples to prevent contamination at all preparation stages. They will have ready access to workplace procedures and will work under direct supervision.This unit applies to technical assistants working in laboratories in the biomedical, environmental, biotechnology and education industry sectors.No licensing or certification requirements exist at the time of publication. However, regulations and/or external accreditation requirements for laboratory operations exist, so local requirements should be checked. Relevant legislation, industry standards and codes of practice within Australia must also be applied.
Notes
Elements and Performance Criteria
1. Work safely according to the legal and regulatory framework
  • 1.1 Identify hazards and workplace controls associated with the sample, preparation methods, reagents and equipment
  • 1.2 Select, fit and use personal protective equipment (PPE)
  • 1.3 Address hazards and incidents as they arise in accordance with workplace procedures
  • 1.4 Maintain a chain of custody, traceable to the worker, for all cells and tissues
2. Prepare and test cell or tissue culture media
  • 2.1 Select and confirm media specifications and processes and methods
  • 2.2 Prepare culture media to suit the application
  • 2.3 Sterilise culture media and check for sterility
  • 2.4 Perform quality control checks to ensure that culture media is fit for purpose
  • 2.5 Store culture media in accordance with specifications
3. Use tissue or cell culture preparation techniques
  • 3.1 Select tissue or cell sample to optimise growth and prepare it for culture
  • 3.2 Add specified growth agents and/or nutrients
  • 3.3 Inoculate culture medium using aseptic techniques
4. Monitor tissue or cell culture
  • 4.1 Incubate culture in specified conditions
  • 4.2 Monitor growth of culture and record appearance and characteristics
  • 4.3 Identify expected cell types and recognise normal and abnormal cells
  • 4.4 Report presence or absence of contamination
  • 4.5 Subculture the culture to continue the cell line
  • 4.6 Dispose of biohazardous and other laboratory waste safely
5. Maintain records
  • 5.1 Maintain accurate records of batches of media
  • 5.2 Complete records of tissue cultures so that they are retrievable, legible and accurate
  • 5.3 Complete records in accordance with workplace and legal requirements
No information
No information
Replaces
State Code National Code Title Type
OAC10 MSL974025 Prepare tissue and cell cultures Unit of competency