Unit of competency Outline

Date retreived
22/07/2026 10:31 AM AWST

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Perform tissue and cell culture techniques

Perform tissue and cell culture techniques

Unit of competency
National Code
MSL975033
State Code
OAC32
TGA Status
Replaced
DTWD Status
Replaced
Current Release Number
1.00
Current Release Date
20/07/2018
View on TGA
State Implementation and Classification
Approved Date
29/11/2018
Field of Education
019909 - Laboratory Technology
Original Release Date
29/11/2018
Nominal Hours
120
Description
This unit of competency describes the skills and knowledge to prepare, maintain and preserve cells and cell lines for a variety of applications, such as large scale culture, production of monoclonal antibodies, production of viral vaccines and amniocentesis studies. Personnel are required to optimise equipment set-up, media and growth techniques. They are required to detect and investigate contamination and take preventative and/or corrective actions under supervision.This unit applies to laboratory technicians and technical officers working in laboratories in the biomedical, environmental, biotechnology and education industry sectors. No licensing or certification requirements exist at the time of publication. However, regulations and/or external accreditation requirements for laboratory operations exist, so local requirements should be checked. Relevant legislation, industry standards and codes of practice within Australia must also be applied.
Notes
Elements and Performance Criteria
1 Interpret and schedule production requirements
  • 1.1 Review client request and confirm quantity and nature of cells, tissue or products
  • 1.2 Select appropriate media, materials, equipment and methods
  • 1.3 Plan parallel work sequences to optimise production
  • 1.4 Maintain a chain of custody, traceable to the worker, for all cells and tissues
2 Work safely according to the legal and regulatory framework
  • 2.1 Identify hazards and workplace control measures associated with the sample, preparation methods, reagents and equipment
  • 2.2 Select, fit and use personal protective equipment (PPE)
  • 2.3 Address hazards and incidents as they arise
  • 2.4 Safely dispose of biohazardous materials and other laboratory waste
3 Assemble and maintain tissue culture equipment
  • 3.1 Assemble, sterilise or decontaminate equipment according to workplace procedures
  • 3.2 Perform pre-use and safety checks in accordance with relevant workplace and operating procedures
  • 3.3 Identify faulty or unsafe components and equipment and report to appropriate personnel
  • 3.4 Decontaminate area and equipment after use
4 Prepare and test cell and tissue culture media
  • 4.1 Confirm media specifications and processes/methods
  • 4.2 Prepare culture media to suit client request
  • 4.3 Sterilise culture media and check for sterility
  • 4.4 Perform quality control checks to ensure that culture media is fit for purpose
  • 4.5 Store culture media in accordance with specifications
5 Obtain, monitor and maintain tissue and cell lines
  • 5.1 Retrieve/obtain the cell lines or tissue sample from fresh or preserved sources and prepare a culture
  • 5.2 Select specified culture media and add any necessary growth agents or nutrients
  • 5.3 Incubate cells/tissue in specified conditions
  • 5.4 Inoculate the media with the specified amount of sample
  • 5.5 Monitor growth of tissue and cell lines and products
  • 5.6 Detect contamination/abnormalities and troubleshoot materials, equipment and techniques
  • 5.7 Passage samples by subculturing to preserve or grow the line
  • 5.8 Harvest cells or cell products to optimise yields
6 Preserve cells and tissues
  • 6.1 Select the appropriate preservation method
  • 6.2 Preserve the cell lines or tissue in accordance with the method
  • 6.3 Check preserved cell lines regularly to ensure viability is maintained
7 Maintain records
  • 7.1 Maintain records of batches of media
  • 7.2 Maintain records of active and stored tissue and cell lines
  • 7.3 Complete records so that they are retrievable, legible and accurate
  • 7.4 Ensure records conform to the information management, records, quality system and legal requirements
No information
No information
Replaces
State Code National Code Title Type
AWN84 MSL975013 Perform tissue and cell culture techniques Unit of competency
Replaced By
State Code National Code Title Type
OEX69 MSL975060 Perform tissue or cell culture techniques Unit of competency