Unit of competency Outline

Date retreived
23/07/2026 4:51 AM AWST

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Perform advanced histological techniques

Perform advanced histological techniques

Unit of competency
National Code
MSL975056
State Code
OEY11
TGA Status
Current
DTWD Status
Approved
Current Release Number
1.00
Current Release Date
20/12/2022
State Implementation and Classification
Approved Date
24/05/2023
Field of Education
019909 - Laboratory Technology
Original Release Date
24/05/2023
Nominal Hours
135
Description
This unit of competency describes the skills and knowledge required to perform procedures associated with embedding tissue and performing microtomy on those tissues in preparation for staining for examination of tissue structure and abnormalities by pathologists and scientists to assist with disease diagnosis.This unit applies to laboratory technicians and technical officers in the biomedical sector. The unit principally refers to techniques performed on human tissues, but many aspects may be relevant to animal and plant tissues. This unit of competency assumes that the technical officer would perform tests and procedures under the close supervision of scientific and/or medical staff. Although a supervisor may not always be present, the technician will follow workplace procedures that will clearly describe the scope of permitted practice in modifying testing and quality control procedures. Technical workers may need to interrupt their routine work in order to assist with or perform procedures to facilitate rapid diagnosis of specimens. No licensing or certification requirements exist at the time of publication. However, regulations and/or external accreditation requirements for laboratory operations exist, so local requirements should be checked. Relevant legislation, industry standards and codes of practice within Australia must also be applied.
Notes
Elements and Performance Criteria
1. Prepare specialised tissues
  • 1.1 Prepare agar plugs for cytological or minute specimens in accordance with workplace procedures
  • 1.2 Prepare blood clot for processing in accordance with workplace procedures
  • 1.3 Prepare friable /tiny tissue in sponges and/or wrapped to ensure tissue integrity and prevent loss during processing in accordance with workplace procedures
  • 1.4 Decalcify bone in accordance with workplace procedures
2. Embed tissue
  • 2.1 Identify and prioritise specimens prior to embedding
  • 2.2 Select appropriately sized mould
  • 2.3 Embed tissue types in correct orientation and characteristics that minimise tissue loss and artefacts during microtomy
  • 2.4 Embed tissue with embedding orientation markings
  • 2.5 Confirm number of tissues is as expected and record any discrepancies
  • 2.6 Inspect blocks, reject and re-embed items that do not meet quality control standards
3. Cut tissue sections
  • 3.1 Prepare and adjust all external parts of a microtome and associated equipment to accommodate requirements of tissue batch
  • 3.2 Orientate the block correctly for each specific tissue in order to maintain sample integrity and minimise artefacts
  • 3.3 Apply microtomy techniques to trim blocks to full face without significant loss of tissue
  • 3.4 Cut and pick up thin tissue sections according to needs of subsequent procedures, maintaining sample integrity, minimising artefacts and conserving tissue for further testing
  • 3.5 Inspect sections and reject any that do not meet quality control standards
  • 3.6 Identify, troubleshoot and resolve common section quality control issues
  • 3.7 Apply quality control procedures to troubleshoot discrepancies between request-sheet and sections on slides
No information
No information
Replaces
State Code National Code Title Type
OAC71 MSL975028 Apply advanced embedding and microtomy skills Unit of competency
State Code National Code Title Type
BIX5 MSL50122 Diploma of Laboratory Technology Qualification