Unit of competency Outline

Date retreived
23/07/2026 1:57 AM AWST

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Perform aseptic techniques

Perform aseptic techniques

Unit of competency
National Code
MSL973004
State Code
AWO20
TGA Status
Replaced
DTWD Status
Replaced
Current Release Number
1.00
Current Release Date
01/03/2016
View on TGA
State Implementation and Classification
Approved Date
16/08/2016
Field of Education
019909 - Laboratory Technology
Original Release Date
16/08/2016
Nominal Hours
40
Description
This unit of competency covers the ability to perform aseptic techniques to maintain the integrity of both the sample source and the sample. It applies to sampling techniques in tissue culture and to generic microbiological procedures.This unit of competency is applicable to laboratory assistants and technicians working in the field or laboratory in the biomedical, biological, food processing and environmental industry sectors.While no specific licensing or certification requirements apply to this unit at the time of publication, laboratory operations are governed by relevant legislation, regulations and/or external accreditation requirements. Local requirements should be checked
Notes
Elements and Performance Criteria
1 Prepare for aseptic sampling or transfer
  • 1.1 Ensure that any sampling procedure conforms with the requirements of the sampling plan
  • 1.2 Use specified personal protective equipment (PPE)
  • 1.3 Prepare the work area for safe and effective sample transfer
  • 1.4 Select equipment and materials specified by the procedure
  • 1.5 Organise equipment to minimise contamination during manipulations
  • 1.6 Label containers for clear identification
  • 1.7 Record details in relevant log or database
2 Transfer materials aseptically
  • 2.1 Protect the integrity of the sample source by sterilising the sampling site and the mouth of transport or culture vessel
  • 2.2 Sterilise inoculating loops and/or pipette where used to prevent contamination
  • 2.3 Perform transfer while minimising opportunities for contamination and cross-infection
  • 2.4 After transfer, and before sealing the transport or culture vessel, flame the vessel mouth to maintain sterility
  • 2.5 Re-sterilise inoculating loops, minimising the generation of aerosols
  • 2.6 Perform quality control checks as required
  • 2.7 Label transport or culture vessels for clear identification
3 Maintain work area and equipment to prevent cross-infection and contamination
  • 3.1 Place disposable and reusable items into relevant receptacles
  • 3.2 Clean and disinfect work area and equipment after use
  • 3.3 Transport disposable and reusable contaminated materials to relevant areas for disinfection, sterilisation and cleaning or disposal
No information
No information
Replaces
State Code National Code Title Type
WA708 MSL973004A Perform aseptic techniques Unit of competency
Replaced By
State Code National Code Title Type
OAC93 MSL973016 Perform aseptic techniques Unit of competency