Unit of competency Outline
Date retreived
22/07/2026 6:42 AM AWST
22/07/2026 6:42 AM AWST
Whilst all efforts are made to provide accurate and timely information from the relevant source/documentation, please be aware that the information supplied may not be the most current version. The accuracy of the detail has not been confirmed by the Department and therefore should not be relied upon without first confirming the contents.
Prepare and pour culture media
Prepare and pour culture media
Unit of competency
National Code
FDFLAB2005A
FDFLAB2005A
State Code
S7955
S7955
TGA Status
Deleted
Deleted
DTWD Status
Transition (Deleted)
Transition (Deleted)
State Implementation and Classification
Approved Date
13/06/2012
Field of Education
019909 - Laboratory Technology
Original Release Date
13/06/2012
Nominal Hours
20
Description
This unit covers the skills and knowledge required to prepare and dispense culture media for microbiological tests in a winery laboratory. Work is usually done under routine supervision.
Notes
Elements and Performance Criteria
1. Prepare materials and equipment
- 1.1 Appropriate materials are selected and confirmed as available for use
- 1.2 Materials are prepared to meet requirements
- 1.3 Work area is prepared to meet requirements
- 1.4 Services are confirmed as available and ready for use
- 1.5 Equipment is selected, prepared and checked to confirm readiness for use
2. Prepare culture media
- 2.1 Media and solvent are mixed thoroughly according to workplace procedures and manufacturer instructions
- 2.2 Media is placed in appropriate vessels for sterilisation
- 2.3 Steriliser is loaded and operated to achieve optimum sterilising conditions for culture media
- 2.4 Sterilisation cycle is monitored to confirm equipment operating condition
- 2.5 Appropriate personal protective equipment is used when removing media from steriliser
- 2.6 Culture media is cooled as required
- 2.7 Out-of-specification process and equipment performance is identified, rectified and/or reported
3. Complete preparation of culture media
- 3.1 Heat labile additions are made using aseptic technique as required
- 3.2 Media is dispensed aseptically as specified
- 3.3 Culture media is stored and labelled according to instructions
- 3.4 Media is monitored to confirm quality specifications are maintained
- 3.5 Equipment and work area are cleaned and/or disinfected after use
- 3.6 Waste generated by the process is monitored and cleared according to workplace procedures
- 3.7 Work is conducted in accordance with workplace environmental guidelines
4. Record information
- 4.1 Workplace information is recorded in the appropriate format
RANGE STATEMENT
The range statement relates to the unit of competency as a whole. It allows for different work environments and situations that may affect performance. Bold italicised wording, if used in the performance criteria, is detailed below. Essential operating conditions that may be present with training and assessment (depending on the work situation, needs of the candidate, accessibility of the item, and local industry and regional contexts) may also be included.
Policies and procedures
Work is carried out in accordance with workplace procedures, licensing requirements and legislative requirements
Workplace information
Workplace information may include:
Standard Operating Procedures (SOPs)
specifications
production schedules
instructions
Material Safety Data Sheets (MSDS)
work notes
manufacturer instructions
verbal direction from laboratory manager, supervisor or senior operator
Culture media
Culture media may include:
agars and broths required for the analyses and techniques performed by the microbiology laboratory
Equipment
Equipment may include:
hot plate/stirrer
autoclave
analytical balance
pH meter
measuring cylinders
volumetric flasks and glassware
media storage vessels
McCartney bottles
test tubes
pipettes
filtration apparatus
Materials
Materials may include:
dehydrated commercial preparations
juices
additives
Petri dishes
cotton wool
aluminium foil
sterilisation indicators
filters
Equipment status
Confirming equipment status involves:
checking that hygiene and sanitation standards, safety standards, pre-start requirements are met and that equipment is operational
checking the operation and/or calibration of measuring instrumentation
Services
Services may include:
water
power
steam
gases
Information systems
Information systems may be:
print or screen based
The range statement relates to the unit of competency as a whole. It allows for different work environments and situations that may affect performance. Bold italicised wording, if used in the performance criteria, is detailed below. Essential operating conditions that may be present with training and assessment (depending on the work situation, needs of the candidate, accessibility of the item, and local industry and regional contexts) may also be included.
Policies and procedures
Work is carried out in accordance with workplace procedures, licensing requirements and legislative requirements
Workplace information
Workplace information may include:
Standard Operating Procedures (SOPs)
specifications
production schedules
instructions
Material Safety Data Sheets (MSDS)
work notes
manufacturer instructions
verbal direction from laboratory manager, supervisor or senior operator
Culture media
Culture media may include:
agars and broths required for the analyses and techniques performed by the microbiology laboratory
Equipment
Equipment may include:
hot plate/stirrer
autoclave
analytical balance
pH meter
measuring cylinders
volumetric flasks and glassware
media storage vessels
McCartney bottles
test tubes
pipettes
filtration apparatus
Materials
Materials may include:
dehydrated commercial preparations
juices
additives
Petri dishes
cotton wool
aluminium foil
sterilisation indicators
filters
Equipment status
Confirming equipment status involves:
checking that hygiene and sanitation standards, safety standards, pre-start requirements are met and that equipment is operational
checking the operation and/or calibration of measuring instrumentation
Services
Services may include:
water
power
steam
gases
Information systems
Information systems may be:
print or screen based
EVIDENCE GUIDE
The evidence guide provides advice on assessment and must be read in conjunction with the performance criteria, required skills and knowledge, range statement and the Assessment Guidelines for the Training Package.
Overview of assessment
Assessment must be carried out in a manner that recognises the cultural and literacy requirements of the assessee and is appropriate to the work performed. Competence in this unit must be achieved in accordance with food safety standards and regulations.
Critical aspects for assessment and evidence required to demonstrate competency in this unit
Evidence of ability to:
prepare equipment and materials for media preparation
prepare media according to workplace requirements
maintain sterilisation standards
identify and act on out-of-standard results
conduct housekeeping and dispose of waste according to workplace standards
complete documentation.
Context of and specific resources for assessment
Assessment must occur in a real or simulated workplace where the assessee has access to:
personal protective clothing and equipment as required
work procedures, including advice on company practices, safe work practices, food safety, quality and environmental requirements
instructions, information, specifications and schedules as required
equipment, services and corresponding information as required
products and materials as required
internal and external customers and suppliers as required
cleaning procedures, materials and equipment as required
documentation and recording requirements and procedures.
Method of assessment
This unit should be assessed together with other units of competency relevant to the function or work role.
Guidance information for assessment
To ensure consistency in one's performance, competence should be demonstrated on more than one occasion over a period of time in order to cover a variety of circumstances, cases and responsibilities, and where possible, over a number of assessment activities.
The evidence guide provides advice on assessment and must be read in conjunction with the performance criteria, required skills and knowledge, range statement and the Assessment Guidelines for the Training Package.
Overview of assessment
Assessment must be carried out in a manner that recognises the cultural and literacy requirements of the assessee and is appropriate to the work performed. Competence in this unit must be achieved in accordance with food safety standards and regulations.
Critical aspects for assessment and evidence required to demonstrate competency in this unit
Evidence of ability to:
prepare equipment and materials for media preparation
prepare media according to workplace requirements
maintain sterilisation standards
identify and act on out-of-standard results
conduct housekeeping and dispose of waste according to workplace standards
complete documentation.
Context of and specific resources for assessment
Assessment must occur in a real or simulated workplace where the assessee has access to:
personal protective clothing and equipment as required
work procedures, including advice on company practices, safe work practices, food safety, quality and environmental requirements
instructions, information, specifications and schedules as required
equipment, services and corresponding information as required
products and materials as required
internal and external customers and suppliers as required
cleaning procedures, materials and equipment as required
documentation and recording requirements and procedures.
Method of assessment
This unit should be assessed together with other units of competency relevant to the function or work role.
Guidance information for assessment
To ensure consistency in one's performance, competence should be demonstrated on more than one occasion over a period of time in order to cover a variety of circumstances, cases and responsibilities, and where possible, over a number of assessment activities.
Replaces
| State Code | National Code | Title | Type |
|---|---|---|---|
| W0913 | FDFLABPCMA | Prepare and pour culture media | Unit of competency |
| State Code | National Code | Title | Type |
|---|---|---|---|
| D684 | FDF30411 | Certificate III in Wine Industry Operations | Qualification |
| D680 | FDF20411 | Certificate II in Wine Industry Operations | Qualification |