Unit of competency Outline

Date retreived
22/07/2026 2:55 PM AWST

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Perform an electrophoresis test on a seed sample

Perform an electrophoresis test on a seed sample

Unit of competency
National Code
AHCSDT408
State Code
BAR62
TGA Status
Replaced
DTWD Status
Replaced
Current Release Number
1.00
Current Release Date
27/06/2016
View on TGA
State Implementation and Classification
Approved Date
12/01/2017
Field of Education
019909 - Laboratory Technology
Original Release Date
12/01/2017
Nominal Hours
90
Description
This unit of competency describes the skills and knowledge required to test seed by separating proteins of varying size and/or electric charge to get a banding pattern that can be used to identify or verify a particular variety.It applies to senior seed analysts who analyse information and exercise judgement to complete a range of advanced skilled activities and who demonstrate deep knowledge in a specific technical area. They have accountability for the work of others and analyse, design and communicate solutions to a range of complex problems. All work is carried out to comply with workplace procedures.No occupational licensing, legislative or certification requirements are known to apply to this unit at the time of publication.
Notes
Elements and Performance Criteria
1. Prepare seed extraction and gel
  • 1.1 Prepare working sample from submitted sample
  • 1.2 Identify seed to be tested and complete records
  • 1.3 Cut or crush single seeds and transfer to centrifuge tubes
  • 1.4 Add extraction solution and mix with crushed seeds and allow to stand for prescribed period
  • 1.5 Centrifuge tubes and collect and store supernatants
  • 1.6 Prepare gel mixture and chemicals and use to achieve required level of polymerisation
2. Carry out electrophoresis
  • 2.1 Place buffer solution in tanks
  • 2.2 Load samples into wells and place gel in tanks
  • 2.3 Carry out electrophoresis at the prescribed voltage and duration
  • 2.4 Maintain temperature as required throughout the process
3. Fix and stain sample
  • 3.1 Remove gel from tank and treat with chemicals as prescribed
  • 3.2 Wash gel and treat to enhance stain
  • 3.3 Examine and/or photograph stain
4. Evaluate stain
  • 4.1 Compare banding pattern with that of an authentic variety or that of a known control sample
  • 4.2 Provide records and reports
  • 4.3 Determine if obvious procedure or equipment problems have led to atypical data or results
5. Maintain a safe work environment
  • 5.1 Use established safe work practices and personal protective equipment
  • 5.2 Minimise the generation of wastes and environmental impacts
  • 5.3 Ensure the safe collection of laboratory and hazardous waste for subsequent disposal
  • 5.4 Care for and store equipment and reagents
6. Maintain laboratory records
  • 6.1 Enter approved data into laboratory information management system
  • 6.2 Maintain confidentiality and security of enterprise information and laboratory data
No information
No information
Replaces
State Code National Code Title Type
WE662 AHCSDT408A Perform an electrophoresis test on a seed sample Unit of competency
Replaced By
State Code National Code Title Type
ODU32 AHCSDT412 Perform advanced tests on seeds Unit of competency
State Code National Code Title Type
BDD6 AHC41516 Certificate IV in Seed Testing Qualification